In Vitro Evaluation of the Antioxidant and Urease Inhibition Activities of Three Triterpenes from the Flowers of Alstonia Scholaris

Nighat Sultana^1* & Muhammad Saleem^1,2

¹Pharmaceutical Research Center, PCSIR Laboratories Complex, Karachi-75280, Pakistan
²Departments of Biotechnology, University of Karachi, Karachi, Pakistan

Dr. Nighat Sultana, Pharmaceutical Research Center, PCSIR Laboratories Complex, Karachi-75280, Pakistan.

Keywords: In Vitro Evaluation; Urease Inhibition; Flowers

Abstract

Reinvestigation of the flowers of Alstonia scholaris of Pakistan origin have resulted in the isolation of three triterpenoids, two of the ursane type, 3 β -acetate- 24-nor- urs-4, 12-diene ester triterpene (1), 3 β -hydroxy- 24-nor-urs-4,12,28-triene triterpene (2) and one of the oleanane type 3,28- β -diacetoxy-5-olea-triterpene (3) together with two known triterpenes α-amyrin acetate (4) and ursolic acid (5). These compounds were analyzed for antioxidant and antiurease inhibition tests. Compounds 2 and 4 were subjected to enzymatic bioassays. Compounds 2 and 4 were found to be antioxidant with IC₅₀ 107±0.39

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